Negative-stain EM structure of the endogenous TREX complex from S. cerevisiae. (A) A representative negative-stain micrograph of the BS3 cross-linked S. cerevisiae TREX complex. Scale bar represents 500 Å. (B) Representative reference-free 2D class averages of the S.c. TREX complex. Scale bar represents 100 Å. (C) Fourier shell correlation (FSC) plot for the negative-stain reconstruction of the S.c. TREX complex. The resolution at FSC = 0.143 (dashed line) and FSC = 0.5 (dotted line) are indicated. (D) Three-dimensional reconstruction of the S.c. TREX complex resolved to 26 Å. (E) Representative views of the Tho–Sub2 dimer structure (PDB ID: 7aqo) in surface representation lowpass-filtered to 25 Å. (F) Color-coded THO–Sub2 dimer PDB model depicted in the same views as in (E). Proteins are color-coded as in Figure 1.
