FIGURE 5.
RNase selection affects IR-PAR-CLIP coverage, motif and target prediction results. (A) Examples of IGF2BP3 PAR-CLIP read coverage of 3′UTRs of TP53, MYC, and HMGA2 transcripts. (B) 4-nt IGF2BP3-binding motifs identified by HOMER in IR-PAR-CLIP of HCT116. (C) Overlap of IGF2BP3 targets identified in this study with RNase I and RNase T1. Concentrations of RNases used for this figure in-lysate and on-beads: RNase I 0.05 U/µL, RNase T1 1 U/µL. (D) Examples of IGF2BP3 PAR-CLIP library-size normalized read coverage of 3′UTRs of PARK7, SRPRB, and FABP5 transcripts. Transitions color code: (A) green, (C) blue, (G) brown, (T) red.
