Optimized infrared photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation (IR-PAR-CLIP) protocol identifies novel IGF2BP3-interacting RNAs in colon cancer cells

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FIGURE 4.
FIGURE 4.

Effect of RNase treatment conditions on the recovery of crosslinked RNA fragments. (A) Read alignment of the libraries made in this study to genome features. (B) Counts and percentages of the unique deduplicated reads in the final libraries depending on RNase treatment conditions. (C) The number of clusters identified with PARalyzer depending on RNase treatment conditions. (D) Distribution of lengths of deduplicated reads within the 18–45 nt range.

This Article

  1. Published in Advance August 15, 2023, doi: 10.1261/rna.079714.123 RNA 29: 1818-1836

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