FIGURE 9.
RNA capping activity of FCE and VCE on structured model RNA. Synthetic oligonucleotides are designed to adopt hairpin structures with different folding energies and availability of the 5′ triphosphate. All RNA oligos contain a FAM-dT for capillary electrophoresis readout and their theoretical minimum free energies (MFEs) of unfolding are listed. Capping reactions were carried out on 0.5 µM of the RNA oligos using 2 nM of FCE or VCE at 37°C under standard conditions (see Materials and Methods). Data points are the average of triplicated reactions. Error bars represent standard deviations.
