FIGURE 8.
RNA capping activity of FCE and VCE across a temperature range. RNA capping assays were performed on 0.5 µM of a 20 nt poly(A) synthetic oligonucleotide with a 3′ FAM group using 5 nM of FCE or VCE under standard conditions (see Materials and Methods). (A) FCE generated more m7Gppp-cap at all temperatures tested. Data points are the average of triplicated reactions. Error bars represent standard deviations. (B) A breakdown of the intermediate products across the temperature range after 30 min.
