Loss of Fyv6 changes 3′ SS selection in yeast. (A) Schematic of the 3′ SS competition reporter (3′ SS comp) showing relative locations of the proximal and distal sites. (B) Images of representative yeast growth on copper-containing media shown after 48 (WT) or 72 h (fyv6Δ) for strains containing the 3′ SS comp ACT1–CUP1 reporter. (C) Maximum copper tolerances observed for each strain for N = 3 replicates (dots). Bars represent the average values. (D) Representative primer extension analysis of mRNAs generated by yeast using the distal (mRNAD) or proximal (mRNAP) 3′ SS in the presence (WT) or absence of Fyv6 (fyv6Δ). U6 snRNA was analyzed as a loading control. (E) Quantification of the primer extension results from N = 3 replicates (dots) expressed as a ratio of mRNAP/mRNAD. Bars represent the average of the replicates ±SD. Means between the two experimental groups were compared with an unpaired Welch's two-tailed t-test (P = 0.04262).
