Biochemical and genetic evidence supports Fyv6 as a second-step splicing factor in Saccharomyces cerevisiae

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FIGURE 3.
FIGURE 3.

Impact of FYV6 deletion on yeast copper tolerance using the ACT1–CUP1 assay. (A) Schematic of the WT ACT1–CUP1 reporter along with intronic substitutions. (B) Images of representative yeast growth on copper-containing media shown after 48 (WT) or 72 h (fyv6Δ) for strains containing the indicated ACT1–CUP1 reporters. (C) Maximum copper tolerances observed for each strain for N = 3 replicates (dots). Bars represent the average values.

This Article

  1. Published in Advance August 25, 2023, doi: 10.1261/rna.079607.123 RNA 29: 1792-1802

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