FIGURE 1.
Sequence alignment of Fyv6 with FAM192 and unassigned EM density in yeast spliceosome structures. (A) Sequence-based alignment of S. cerevisiae Fyv6 and human FAM192A using EMBOSS Needle (Needleman and Wunsch 1970). (B,C) Superposition of the atomic models for the spliceosome C* (panel B, 5MQ0) and P (panel C, 6BK8) complexes with the unassigned EM density shown in blue spacefill. The three putative Fyv6 α helices identified by Zhan and coworkers are annotated next to the corresponding EM density in panel B. Images were prepared using ChimeraX (Pettersen et al. 2021). (D) Impact of fyv6Δ on yeast growth at various temperatures. Plates were imaged on the days shown.
