Effect of the SARS-CoV-2 Delta-associated G15U mutation on the s2m element dimerization and its interactions with miR-1307-3p

(A) One-dimensional ¹H NMR spectroscopy of the SARS-CoV-2 s2m G15U (top) and SARS-CoV-2 s2m reference (bottom). The labeled resonances were assigned previously for the s2m reference and in the current work for s2m G15U (Wacker et al. 2020; Imperatore et al. 2022). (B) ¹H–¹H NOESY spectra of the imino proton resonances of s2m G15U with labeled NOEs between base paired U imino and A H2 and between G imino protons and neighboring A H2. (C) ¹H–¹H NOESY spectra of the imino proton resonances for the s2m G15U with labeled NOE between neighboring intrastrand and interstrand Gs and Us.