Fission yeast poly(A) polymerase active site mutation Y86D alleviates the rad24Δ asp1-H397A synthetic growth defect and up-regulates mRNAs targeted by MTREC and Mmi1

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FIGURE 1.
FIGURE 1.

Genetic modulation of transcriptional interference with pho1 expression. (A) lncRNA transcription across the pho1 mRNA promoter interferes with pho1 mRNA synthesis. Genetic maneuvers that diminish the probability of lncRNA 3′-processing/termination prior to the mRNA promoter increase interference and hyper-repress pho1 mRNA expression. Genetic backgrounds in which Pho1 expression is hyper-repressed are shown at right. (B) Precocious termination of lncRNA synthesis in response to poly(A) signals well upstream of the pho1 mRNA promoter can result in derepression of pho1 mRNA transcription. Genetic backgrounds in which Pho1 expression is derepressed are shown at right.

This Article

  1. Published in Advance August 16, 2023, doi: 10.1261/rna.079722.123 RNA 29: 1738-1753

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