In vitro methylation of the U7 snRNP subunits Lsm11 and SmE by the PRMT5/MEP50/pICln methylosome

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FIGURE 4.
FIGURE 4.

Methylation of SmE by recombinant methylosome. (A–C) Recombinant PRMT5/MEP50 complex was incubated with indicated Sm or Lsm heterodimers and 3H SAM either in the absence or presence of pICln and SmE/F/G heterotrimer, as indicated. Protein methylation was detected, as described in the legend for Figure 2. Coomassie Blue stained gels and fluorograms are shown at the top and bottom, respectively.

This Article

  1. Published in Advance August 10, 2023, doi: 10.1261/rna.079709.123 RNA 29: 1673-1690

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