Comparison of 16S rRNA DMS reactivity in methanol and acetate. DMS reactivity comparison in acetate and methanol for (A) Helix 44 and (B) Helix 27. Both panels are oriented as in the full 16S rRNA diagram found in the Supplemental Information. Colored A and C nucleotides have significant DMS reactivity (see legends). Helices are numbered according to standardized format and nucleotides have a tick every 10 nt. (C) Correlation plot of acetate and methanol DMS reactivity. Line indicates y = x. (D) DMS reactivity difference plot. Each bar was calculated by DMS reactivity in methanol − acetate. Green bars indicate higher DMS reactivity in methanol, whereas orange bars indicate higher DMS reactivity in acetate. Select bars are annotated for reference.
