In vivo structure probing of RNA in Archaea: novel insights into the ribosome structure of Methanosarcina acetivorans

Comparison of 5S rRNA DMS reactivity in methanol and acetate. DMS reactivity in (A) acetate, (B) methanol, and (C) methanol–acetate. Colored A and C nucleotides have significant DMS reactivity (see legends). Helices are numbered according to standardized format, and nucleotides have a tick every 10 nt. (D) Correlation plot of acetate and methanol DMS reactivity. Line indicates y = x. (E) DMS reactivity difference plot. Each bar was calculated as DMS reactivity in methanol − acetate. Green bars indicate higher DMS reactivity in methanol, whereas orange bars indicate higher DMS reactivity in acetate. Select bars are annotated for reference.