Impacts of A3 mutations on RNA editing. (A) qPCR measurements of pre-edited and edited mRNAs as indicted in samples from BF and PF cells grown in the presence or absence of tet for 48 and 96 h, respectively. The levels of RNAs were normalized to the TERT control. Relative abundances of mRNAs (−tet vs. +tet) were then determined for each sample, transformed by log10, and represented by a heat map. The measurements are representative of up to three biological replicates with two technical replicates for each; see Materials and Methods for details. (B) Gel profiles of RT-PCR products with pre-edited (Pre) and fully edited (Ed.) sizes as indicted are from cells grown for 48 h (BF) or 96 h (BF with an MGA allele or PF) following tet removal. Only minus tet samples are shown unless indicated otherwise. (Box) Selected PF cell lines that represent different regions of the OB-fold; most mutations do not result in growth defects in PF. The profiles shown represent two biological replicates for the CN-background cell lines and one replicate for the MGA cell lines.
