Autorecoding A-to-I RNA editing sites in the Adar gene underwent compensatory gains and losses in major insect clades

The evolutionary trajectory of the linkage of the two autorecoding sites. Editable codons are colored in red and uneditable codons in blue. Site1 in Diptera underwent compensatory mutations to acquire an editable Ser codon, which compensated the uneditable Ile in site2. As the postedited amino acids (Gly at site1 and Met at site2) did not appear at the ancestral nodes, these two autorecoding sites did not conform with the restorative hypothesis mentioned below.