The DUF3715 domain arose in early eukaryotes and is restricted to orthologs of TEX15, TASOR, and TASORB in vertebrates. Phylogenetic analysis of DUF3715. (A) DUF3715-containing protein architectures are present across the metazoan tree of life, TASOR-like denotes architectures that contain DUF3715 followed by the SPOC–DomI domains as found in TASOR. (B) Unrooted maximum likelihood tree of DUF3715 sequences found across the tree of life. The vertebrate clades are shown in color, and invertebrate clades in black. The presence of the SPOC–DomI domains is indicated by red squares. Positions of human TASOR and human and zebrafish TEX15 DUF3715 domains used in the reconstitution experiment are indicated with black stars. Distance is average substitutions per site, bootstrap support over 80% is indicated as dots.
