FIGURE 3.
Knockdown of Gtsf1 and Gtsf1L decreases Masc and Fem piRNAs, respectively. (A) Normalized reads of 1U (but not 10A) or 10A (but not 1U) small RNA from BmN4 cells transfected with dsRNA for Renilla luciferase (Rluc, control), Gtsf1 or Gtsf1L. (B) Box plots showing the expression change of 1U (but not 10A) and 10A (but not 1U) piRNAs by knockdown of Gtsf1 or Gtsf1L relative to control knockdown in BmN4 cells. Center line, median; box limits, upper and lower quartiles; whiskers, 1.5 × interquartile range; points, outliers. (C) Schematic representation of the production of Fem and Masc piRNAs via the ping-pong cycle (left). The expression of Fem and Masc piRNAs in Gtsf1- or Gtsf1L-knockdown (right).
