The two Gtsf paralogs in silkworms orthogonally activate their partner PIWI proteins for target cleavage

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 2.
FIGURE 2.

Gtsf1 and Gtsf1L show increased association with catalytically inactive Siwi and BmAgo3, respectively. (A) Western blot analysis of the interaction between Gtsf1-FLAG and HA-tagged wild-type (WT) Siwi or catalytically inactive Siwi-D670A. (B) Subcellular localization of Gtsf1-FLAG coexpressed with mCherry-Siwi or -Siwi-D670A. Scale bar, 5 µm. (C) Subcellular localization of Gtsf1-FLAG coexpressed with mCherry-Siwi or -Siwi-D670A, and HA-Dcp2, a P-body marker. Scale bar, 5 µm. (D) Western blot analysis of the interaction between Gtsf1L-HA and FLAG-tagged wild-type (WT) BmAgo3 or catalytically inactive BmAgo3-D697A. (E) Subcellular localization of Gtsf1L-HA coexpressed with mCherry-BmAgo3 or -BmAgo3-D697A. Scale bar, 5 µm.

This Article

  1. Published in Advance November 1, 2022, doi: 10.1261/rna.079380.122 RNA 29: 18-29

Current Issue

  1. August 2026, 32 (8)
  1. Current Issue
  1. Alert me to new issues of RNA