Effects of DRBD18 RNAi on the RNAs from Tb927.8.1580 and Tb927.8.1590. (A) Results from the Integrated Genome Viewer in the region containing Tb927.8.1580 and Tb927.8.1590. The direction of transcription and a scale bar are at the top. Representative views for all six types of samples are shown. The scales on the y-axis are different (see numbers on the right); the thin lines are drawn at intervals that show read depths of approximately 100. A map of the detected mRNAs is below, as for Figure 1. (B) Effects of RNAi on coding region reads (log2 fold change from DeSeq2). (C) Method to estimate the relative abundances of wild-type and alternatively processed reads. For more detail, see Supplemental Figure S5. Reads that spanned the sites were designated as wild-type, and those with polyadenylation or spliced leader sequences were classified as alternatively processed. “All”—whole-cell RNA; Cyt: cytoplasmic RNA. (D) The upper two bar graphs show numbers of reads (normalized to total data set size) that were either wild-type or alternatively processed at the different poly(A) sites; all of these events are directed by trans splicing at a single downstream site, results for which are shown in the bottom panel. The dot plots show the proportions of mRNA that were alternatively processed (A = All). (E) Reads per million for the coding regions of Tb927.8.1580 and the downstream gene, Tb927.8.1590, splicing of which directs wild-type Tb927.8.1580 polyadenylation. Results for all three replicates are shown for each data set.
