FIGURE 5.
DHX34 regulates pre-mRNA splicing of its own pre-mRNA in K562 cells. (A) Schematic of DHX34 protein domain structure, including the D279A catalytic mutation. Part of the DHX34 pre-mRNA exon–intron structure, including the alternative exon 12b harboring a PTC, is depicted. (B) RT-PCR analysis of splicing patterns of DHX34 exon 12b in knockdown and mutant conditions. RT-PCR products were resolved using Bioanalyzer (top panel) and relative splicing changes (PSI) were quantified.
