DHX34 binds in the proximity of exon–intron boundaries. (A) Western blot showing the samples used for the generation of the CLIP sequencing library. In each case, the band migrating at the size that corresponds to DHX34–GFP–FLAG was gel extracted and the RNA fragments were eluted. RNA sequencing libraries were constructed following the seCLIP protocol. (B) Pie chart showing the distribution of DHX34 CLIP reads among different transcript species. (C) Metagene analysis of DHX34 RNA binding. DHX34 binding peaks map mostly to introns and exons. Only a few peaks were found in 5′UTR and 3′UTR. (D) Intronic and exonic peaks overlap, suggesting that DHX34 binding spans splice junctions. (E) Pile-up of a subset of DHX34 bound RNA reads at the exon–intron boundary.
