Biological solution conditions and flanking sequence modulate LLPS of RNA G-quadruplex structures

Thermal denaturation of (G₃A₂)₄ in the presence of nucleotide derivatives. We tested the effects of 0.28 mM AMP, 9.6 mM ATP, 8.3 mM UTP, 2.7 mM cytosine, 2.7 mM cytidine, 0.36 mM CMP, 2.7 mM CMP, and 2.7 mM CTP, and 2.7 mM pyrophosphate. All samples were in the background of 10 mM HEPES (pH 7.5) and K₁₅₀N₁₀M_0.5. Melts were monitored at 294 nm with a heating rate of 0.5°C/min and were internally normalized to the first data point at 20°C. All samples were buffer corrected. Legend is color-coded and arranged in the same order as the individual traces.