The Bacillus subtilis open reading frame ysgA encodes the SPOUT methyltransferase RlmP forming 2′-O-methylguanosine at position 2553 in the A-loop of 23S rRNA

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FIGURE 5.
FIGURE 5.

Purified 70S and 50S ribosomal particles are poor substrates of YsgA. A total of 0.5 A260 units of 70S or 50S ribosomal particles were incubated with 1 µg of purified YsgA and 1 µCi [methyl 3H] SAM. Incorporated radioactivity was measured by scintillation counting. RNA fr50S or fr70S are for RNA extracted from 50S or 70S ribosomal particles, respectively.

This Article

  1. Published in Advance June 16, 2022, doi: 10.1261/rna.079131.122 RNA 28: 1185-1196

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