The Bacillus subtilis open reading frame ysgA encodes the SPOUT methyltransferase RlmP forming 2′-O-methylguanosine at position 2553 in the A-loop of 23S rRNA

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FIGURE 4.
FIGURE 4.

Localization of the YsgA target nucleoside by mass spectrometry. (A) MS/MS sequencing spectra of 2249.3 Da (m/z 1124.13, z = 2−), sequence CU[Gm] UUCGp, obtained from the analysis of B. subtilis wild-type 23S fragment RNase H 2515-2571 after T1 digestion. CID fragmentation shows methylation of G 2553. (B) MS/MS sequencing spectra of 1279.1 Da (m/z 639.07, z = 2−), sequence UUCGp, obtained from the analysis of B. subtilis ΔysgA 23S fragment RNase H 2515–2571 after T1 digestion.

This Article

  1. Published in Advance June 16, 2022, doi: 10.1261/rna.079131.122 RNA 28: 1185-1196

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