Novel, nonannotated TE-embedded miRNAs are present in child and adolescent brains. (A) IGV visualization of nonannotated TE-embedded miRNAs with classical 22 bp peaks in four childhood (blue) DFC BAM files and four adolescent (orange) DFC BAM files, alongside TE and gene annotations for hg19. (B) IGV visualization of three AGO2 RIP-seq (RIP; green) and three input (In; gray) BAM files from adult brain (Petri et al. 2019; GSE106810) for the corresponding region in A. (C) miRNA hairpin schematics from miRNAfold (Tav et al. 2016) for the DNA sequences in A. Twenty-two bp peaks are highlighted by the black bars on both arms of the hairpin. (D) MULTIZ alignment from the UCSC Genome Browser of the 22 bp miRNA sequence beneath the largest peak in A (Blanchette et al. 2004; Navarro Gonzalez et al. 2021). The putative seed region and orientation are indicated.
