FIGURE 9.
The effect of pseudouridine on RNase H cleavage. (A) A Cypridina luciferase transcript (CLuc; 1.8 kb) was cleaved using Tth RNase H in conjunction with CLucTO-26, which directs RNase H, a cleavage site containing a uridine residue (Supplemental Fig. 6). The size of the cleavage products and cleavage sites are indicated. (B) When unsubstituted, median cleavage frequency at the C|UC site was 73% (25 nt) and at the CU|C site was 27% (26 nt). When all uridines were substituted with pseudouridine, cleavage at the C|ΨC site decreased to a median frequency of 34%, with 66% cleavage at the CΨ|C site.
