FET family proteins redundantly rescue the formation of paraspeckles in MEF lacking the expression of mFus. (A) Simultaneous detection of mNeat1_2, exogenously expressed FLAG-tagged FET family proteins, and the paraspeckle marker mNono in MEF derived from mFus KO mice. Note the absence of paraspeckle formation in mFus KO MEF and the rescue of the paraspeckle formation in the cells expressing FET family proteins. The cytoplasmic signals in the green channel (mNeat1_2 channel) were derived from cross-reactions of streptavidin to endogenous biotinylated proteins in the mitochondria. (B) Merged images of exogenously expressed FLAG-tagged FET family proteins (blue), mNeat1_2 (green), and paraspeckle marker mNono (red), and the intensity profile plots. Yellow lines indicate the region analyzed in the intensity profile plots. X-axis represents the number of pixels and y-axis represents arbitrary units for the intensity of the signals of each channel. Scale bars, 10 µm.
