Comparison of dinucleotide frequencies of human ISGs and IFN genes with the bulk human mRNA transcriptome. A comparison of (A) CpG and (B) UpA dinucleotide representation in human ISGs and interferon genes with the bulk human mRNA transcriptome (of coding sequence lengths ≥450 bases). CpG and UpA representations (observed frequency/frequency expected base on mononucleotide frequencies) of IFN-α paralogs were compared with human mRNA sequences in the G + C content range spanning the former (40.2%–50.0%) using an independent samples t-test. CpG and UpA representations in ISGs and mRNA were compared using regression analysis (see Results text; values for individual ISG data sets are shown in Table 2). Sources of ISG sequences: Set 1: Schoggins et al. (2014); Set 2: Burke et al. (2019); Set 3: Shaw et al. (2021).
