Organization of minicircle cassettes and guide RNA genes in Trypanosoma brucei

Association between cassette size (from 5′ end of forward 18 bp repeat to 3′ end of reverse 18 bp repeat) and gRNA type and expression status. Long-dashed lines: medians, short-dashed lines: first and third quartiles. Cassette sizes and standard deviations in brackets are as follows: expressed canonical: 142.6 nt (3.6 nt), expressed noncanonical: 140.2 nt (4.8 nt), nonexpressed canonical: 138.1 nt (5.8 nt), and nonexpressed noncanonical 136.4 nt (5.1 nt).