The iron-chelator DFO increases lariat levels in lymphoblastoid cells. (A) Genome browser screen capture showing raw read counts for the SUDS3 intron 6, an example of a lariat that is elevated in DFO-treated cells. The read density fall-off at the 3′ and 5′ splice sites is characteristic of intron lariats. (B) Lariat enrichment, calculated from branchpoint-traversing reads, is shown for duplicate samples of DFO-treated lymphoblastoid cells and controls. DFO treatment elevates lariats approximately twofold. (C) Plot of log2 fold change in lariat abundance of DFO-treated versus untreated controls cells. (D) DFO treatment does not significantly change DBR1 expression level, FDR-adjusted P-value = 0.18. (E) DFO and EDTA inhibit Dbr1 in a time-dependent manner, with 67% and 38% inhibition, respectively, after 3 h.
