Metal content and kinetic properties of yeast RNA lariat debranching enzyme Dbr1

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FIGURE 3.
FIGURE 3.

Reconstitution of apo-yDbr1 with Fe2+, Zn2+, and Mn2+. (A) Apo-yDbr1 (1 µM) was reconstituted with 0–100 µM of the indicated metals under anaerobic conditions. Apo-yDbr1, black diamonds at metal = 0, had no detectable activity without exogenous metals. (B) Results of curve fitting to obtain estimates of kcat and Kmetal.

This Article

  1. Published in Advance April 22, 2022, doi: 10.1261/rna.079159.122 RNA 28: 927-936

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