Metal content and kinetic properties of yeast RNA lariat debranching enzyme Dbr1

Homology model of yeast Dbr1. (A) The yDbr1 homology model was calculated from EhDbr1 PDB 5k73. The α-pocket contains a Zn²⁺ ion, and the β-pocket contains an Fe²⁺ ion. All seven metal ligands are identical between EhDbr1(gray backbone) and yDbr1 (tan backbone). The OH⁻ nucleophile is the red sphere. (B) Summary of the metal content of all EhDbr1 structures in the PDB. The metals found in the α and β pockets are indicated, along with PDB ID, and a description of the unique ligand if present.