iLIME-seq detects 3′-end modifications in different pre-miRNA isoforms. (A) iLIME-seq detects different modifications at the 3′-end of endogenous pre-miRNAs. The four types of modifications (A, U, G, and C) at the 3′-end of 10 pre-miRNAs (shown in Fig. 4) were sequenced by iLIME-seq. (B) Modifications at the 3′-end of pre-let-7a-3 isoforms are shown as a representative example. (C) The 3′-end uridylation of different pre-miRNA isoforms. The percentage of uridylation (including mono-uridylation, di-uridylation, triuridylation, and oligouridylation), was estimated for each pre-miRNA isoform. (D) The 3′-end uridylation of pre-mir-142 and pre-mir-342 isoforms is shown as representative examples. Primer sequences used for the pre-miRNA sequencing library are shown in Supplemental Table S2.
