Global SLAM-seq for accurate mRNA decay determination and identification of NMD targets

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FIGURE 3.
FIGURE 3.

Transcripts representing common structural classes known to be regulated by NMD. Background subtracted and chase onset normalized T-to-C conversion rates were fitted to first order exponential decay. YML002W: nonsense codon-containing in this strain background. EST3: translational frame-shifting. CPA1: uORF-containing. COX19: long 3′-UTR. Error bars represent the standard deviation. Error bars for YML002 in upf3Δ were not added since it was calculated from two replicates.

This Article

  1. RNA 28: 905-915