(A) Heatmap depicting the fraction of cells classified in the indicated pattern, for the different genes analyzed by the automated pipeline. (B) Impact of treatment with translational inhibitor puromycin on the number of detected RNA clusters. HMMR shows a similar number of clusters, while all other genes have significantly fewer, indicating an implication of translation in cluster formation. (C) Proportion of mRNAs within 2000 nm of a centrosome. Distance threshold was empirically defined as the typical distance between clustered RNAs and the centrosomes. Compared are untreated cells, and cells treated with two different translation inhibitors: cycloheximide, blocking ribosome elongation, or puromycin, inducing premature chain termination. BICD2 has a centrosomal localization pattern, while TRIM59 is a negative control with a random intracellular localization. Results are displayed with different treatments.
