Evasion to NSP1-mediated translation inhibition with NSP1 variants containing mutations in the middle of NSP1. (A) Curves showing relative light unit (RLU) measured after translation in RRL in the absence or presence of 0.1, 0.2, 0.3, 0.4, or 0.5 µM of R77L/T78S/A79T/P80N, (B) V84K/M85V, (C) R77L/T78S/A79T/P80N/V84K/M85V, (D) L92M/E93D, (E) E102I, and (F) L92M/E93D/E102I. The averages are obtained from four independent experiments. Standard deviations or translational activity for each transcript are shown and calculated from four independent experiments. ns: nonsignificant; (*) 0.05 > P-value >0.01; (**) 0.01 > P-value >0.001; (***) P-value <0.001; based on Student's t-test. To facilitate comparisons between NSP1 proteins, the results have also been presented according to the hairpin SL1 tested (Supplemental Fig. S11).
