The Pet127 protein is a mitochondrial 5′-to-3′ exoribonuclease from the PD-(D/E)XK superfamily involved in RNA maturation and intron degradation in yeasts

The homozygous ΔCapet127 strain and functional mutants are viable and do not show respiratory deficiency. Growth on solid agar media containing glucose (YPD) or glycerol (YPG) as the carbon source. A series of 10× dilutions from overnight YPD starter cultures of wild-type (BWP17), heterozygous CaPET127/ΔCapet127 (Δpet127HZ), homozygous ΔCapet127 (ΔCapet127), and two independent point mutant Capet127_D375A/Capet127_D375A (D375A) strains were spotted on plates and incubated for 48 h at 30°C or 37°C.