Identification and mapping of post-transcriptional modifications on the HIV-1 antisense transcript Ast in human cells

Agarose gel electrophoresis of Ast RNA. Total RNA was extracted from Jurkat-AST cells, and Ast RNA was enriched using streptavidin magnetic beads (lane 3). Total RNA (5 and 1 µg, lanes 1,2) and streptavidin-enriched (1 µg, lane 3) RNA samples were then run on a 1.2% agarose gel. The slow-migrating band in the streptavidin-enriched lane is consistent with the expected size of the full-length Ast RNA. The fast migrating band is likely a splice variant of Ast that has been described previously (Kobayashi-Ishihara et al. 2012).