Effects of transgene insertion loci and copy number on Dnmt3L gene silencing through antisense transgene-derived PIWI-interacting RNAs

Bisulfite sequencing of Dnmt3L promoter. The top panel shows a diagram of the Dnmt3L promoter and its methylation positions (white circles). Methylation levels of the Dnmt3L promoter in germ cells of 0-d-old (P0) nontransgenic (Non-Tg) mice and of mice with a transgene cassette inserted at the B3 locus (Tg-B) as well as in those of 10-d-old (P10) non-Tg mice and mice with transgene cassettes inserted at the E1 locus (Tg-E) and at both the E1 and B3 loci (Tg-BE). Black and white circles indicate methylated and unmethylated CpGs, respectively.