Bisulfite sequencing and northern blot of DNMT3L de novo DNA methylation targets. (A) Methylation levels of retrotransposon genes (L1A, LINE1 type A; L1Tf, LINE1 Tf; and IAP, IAP1Δ1) in germ cells of non-Tg and mice with transgenes inserted at the E1 locus (Tg-E), B3 locus (Tg-B), and B3 and E1 loci (Tg-BE). (B) Methylation levels of paternally imprinted genes (Rasgrf1, H19, and Dlk1). (C) Methylation levels of Snrpn, a maternally imprinted gene, representing sorted cell purity. Black and white circles indicate methylated and unmethylated CpGs, respectively. (D) Northern blotting of LINE1 and IAP in testes of 3 wk in the non-Tg and Tg mice. To compare the amount of RNA loaded, the filters were stained with methylene blue after transfer to show the amount of 28S and 18S.
