Transgene insertion sites on chromosome 18 (ch18) of the mouse genome. (A) The four transgene insertion sites found on ch18 and the sequences of the junctions are shown. The numbers represent the location on the genome (NCBI/mm10 assembly). Genomic sequences and unknown sequences are shown in black and green letters, respectively. Transgene sequences are highlighted in blue. (B,C) Read counts around the insertion loci were determined using whole-genome sequencing. The overall lengths of B and C are 300 kb for Ch18qB3 and 10 kb for Ch18qE1, respectively. The read counts show a 100-bp window. The regions flanked by the insertion sites of the transgenic cassette in both B3 and E1 regions have a high genomic read count (red arrow). This indicates a model in which the transgene is sandwiched between duplicated genomic regions.
