Poly(A)-tail measurements provide new insights. (A) Violin plot showing the absolute difference in poly(A)-tail measurements between ONT replicates. (B) Distribution of median poly(A)-tail length across both ONT replicates. Only mRNAs with a combined minimum of 10 reads are included. Median is 69 nt. (C) Distribution of poly(A)-tail lengths for reads aligning to GL50803_40591. (D) As in C but for GL50803_10311. (E) Comparison of poly(A)-tail length between mRNAs encoding ribosomal proteins (median 56.4 nt) and all other mRNAs (median 69.0 nt). Only genes with a minimum of 10 ONT reads were selected for this analysis. (F) GEO enrichment terms for genes with short (orange) or long (blue) poly(A) tails. Only genes with a minimum of 10 ONT reads were selected for this analysis.
