A model for DHX15 mediated disassembly of A-complex spliceosomes

Reduction of DHX15 stabilizes the ATP-independent interaction between U2 snRNP and minimal intron. (A, top) Representative native gel analysis of in vitro spliceosome assembly with a radiolabeled A^min-substrate in control (Mock) and DHX15-immunodepleted (dDHX15) nuclear extracts at the indicated timepoints. (Bottom) Normalized band intensity relative to the entire lane from three independent trials. Statistical differences were examined by paired Student's t-test. (B, top) Representative native gel analysis of in vitro spliceosome assembly with A^min-substrate with different added NTPs at the indicated timepoints. Statistical differences were examined by unpaired Student's t-test.