Structure of Traffic jam (Tj) mRNA and SHAPE analysis of Tj-cis RNA. (A) The Tj-cis element is located in the 3′-UTR of the Tj mRNA. The element is specifically recognized by Yb protein, and piRNA is generated from the 3′ side of the element. (B) A single-stranded region with structural fluctuation was detected by the effect of NMIA for the RNA corresponding to the Tj-cis element, Tj-cis RNA. Colored bars indicate regions affected by NMIA; the 4–16, 21–25, 33–37, 45–50, 58–64, and 66–69 regions are indicated by red, orange, yellow, green, blue, and purple, respectively. An intense band was observed for U23. Residues at the 3′ side of position 75, indicated by black bars, were not clearly analyzed.
