Alternative splicing regulation of membrane trafficking genes during myogenesis

PTBP1 and QKI regulate alternative splicing in membrane trafficking genes. (A) PTBP1 was depleted in myoblasts, and western blot assays for PTBP1 and PTBP2 were performed (top), and the PTBP1 blot was quantified (bottom). (B,C) Splicing of several trafficking genes upon PTBP1 knockdown was evaluated by RT-PCR and quantified by densitometry. (D) QKI was depleted in myotubes, and western blot assays were performed (top) and quantified (bottom). (E,F) Splicing of several trafficking genes upon QKI depletion was evaluated by RT-PCR and quantified by densitometry. Results are shown as mean ± SEM, n = 3, (*) P < 0.05, Welch's t-test. (bp) Basepair, (KDa) kilodalton, (LMNA) lamin A/C, (nt) nucleotide.