Alternative splicing regulation of membrane trafficking genes during myogenesis

The expression of RBPs is regulated during myogenesis and influences splicing of membrane trafficking genes. (A) Quantification of RBP expression during myogenesis. Results are shown as mean ± SEM, n = 6, (*) P < 0.05, Welch's t-test. (B–I) PTBP1 (B), PTBP2 (C), RBFOX2 (D), QKI (E), CELF1 (F), CELF2 (G), MBNL1 (H), and MBNL2 (I) expression in myoblasts (Mb) and myotubes (Mt) was measured by western blotting. (J) Heatmap of splicing changes in trafficking genes upon RBP knockdown that were evaluated by RT-PCR assays and quantified by densitometry. ΔPSI was defined as the difference between the PSI in the knockdowns and the PSI in the control si-RNA. n = 3. (PSI) Percent spliced in.