Membrane trafficking genes are regulated by alternative splicing in a tissue-specific manner and during C2C12 cell differentiation. (A–D) Alternative splicing of trafficking genes in mouse tissues was evaluated by RT-PCR and quantified by densitometry. Heatmap shows the summary of the results of 11 events in adult mice (A). Striated muscles are labeled in red. Three examples are shown: Trip10 (B), Gapvd1 (C), and Tmed2 (D) in neonatal (postnatal day 4.5, p4.5) and adult (ad) tissues. (E,F) Splicing of trafficking genes was evaluated during C2C12 cell differentiation. The heatmap summarizes the results of all the tested events (E). Splicing events in Trip10, Tmed2, and Gapvd1 pre-mRNAs are shown as examples (F). (G) Correlation plot between the PSI in myotubes and the average of adult skeletal gastrocnemius muscle and adult ventricle. Results are shown as mean ± SEM, n = 3, (*) P < 0.05, Welch's t-test, adult versus p4.5 or differentiated (D) versus undifferentiated (UD). (PSI) Percent spliced in, (EDL) extensor digitorum longus, (FDB) flexor digitorum brevis, (TA) tibialis anterior.
