Nuclear magnetic resonance reveals a two hairpin equilibrium near the 3′-splice site of influenza A segment 7 mRNA that can be shifted by oligonucleotides

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FIGURE 4.
FIGURE 4.

NMR 2D TOCSY spectrum of 3 mM HP1 at 25°C with mixing time of 29 msec in D2O. H1′–H2′ cross-peaks are labeled with corresponding residue. The magnitude of the H1′–H2′ scalar-coupling constant (3J) determined from splitting of H1′ peaks in a 400 msec mixing time NOESY spectrum is given below the residue identifier. Coupling accuracy is approximately ±0.5 Hz.

This Article

  1. Published in Advance January 4, 2022, doi: 10.1261/rna.078951.121 RNA 28: 508-522

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