Nuclear magnetic resonance reveals a two hairpin equilibrium near the 3′-splice site of influenza A segment 7 mRNA that can be shifted by oligonucleotides

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FIGURE 3.
FIGURE 3.

1D imino proton spectra at 1°C of 1.5 mM HP1 (A), 0.5 mM HP2 (B), 0.5 mM HP2 + Oligo1 (C), 0.5 mM HP2 + Oligo2 (D), and 0.5 mM HP2 + Oligo3 (E). Sharp peak at 10.35 ppm in B is likely due to G6 in GAAA tetraloop of HP2-HP (Chen et al. 2015). Buffers are 0.05 mM Na2EDTA (pH 6.2) with 2 mM potassium phosphate for HP1 and 10 mM potassium phosphate for solutions with HP2.

This Article

  1. Published in Advance January 4, 2022, doi: 10.1261/rna.078951.121 RNA 28: 508-522

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