AMT–NHS and UV crosslink Cbf5 to different sites on H/ACA snoRNAs. (A) Crosslinking sites on snR8. The secondary structures and sequence motifs are labeled. Crosslinking sites are shown as circles colored by crosslinking approach. (B) Distribution of crosslinking sites on H/ACA snoRNA secondary structures. Others refer to large insertions present in several snoRNAs. Each crosslinking site is denoted by a circle whose radius is proportional to mut/del rates. The sequences around the H and ACA motifs are shown and the mut/del sites of UV crosslinking are colored magenta. (C) Venn plot of crosslinking sites in UV, AMT–NHS in vivo and AMT–NHS in vitro crosslinked H/ACA snoRNAs. (D) Venn plot of crosslinking sites in H/ACA snoRNAs from UV crosslinked samples and an untreated sample.
